Isolation and characterization of a photoreceptor gene MPD1

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  • English
National Library of Canada , Ottawa
SeriesCanadian theses = -- Thèses canadiennes
The Physical Object
Pagination2 microfiches : negative.
ID Numbers
Open LibraryOL22469032M
ISBN 100612631354

Isolation and characterization of a photoreceptor gene MPDl Degree of Master of Science, Martina Puchyr Graduate Department of Molecular and Medical Genetics. University of Toronto ABSTRACT The majority of genes associated with retinal degenerations are expressed specifically in photoreceptor cells.

The Drosophila retinal degeneration B protein (RdgB) is a novel integral membrane phosphatidylinositol transfer protein required for photoreceptor cell viability and light response. We isolated one intragenic suppressor (rdgBsu) and four autosomal suppressors of the hypomorphic rdgBKS retinal degeneration by: We conducted a molecular characterization of the rdgB gene to examine the nature of the gene product.

Isolation and characterization of a photoreceptor gene MPD1 book Through the isolation and analysis of X-ray-induced rdgB alleles, the cytogenetic position of the gene was determined to be the 12C1 salivary region.

Genomic DNA corresponding to this region was isolated by a chromosomal by: Isolation and characterization of the presumed photoreceptor protein of Blepharisma japonicum Article in Photochemistry and Photobiology 58(2) - January with 10 Reads.

Photosynthetic stramenopile have chloroplasts of secondary endosymbiotic origin and are significant as aquatic primary productivity and biomass production. In marine environments, many photosynthetic stramenopiles utilize blue light to regulate growth, development, and organelle movement. Aureochrome (AUREO) is a new type blue light photoreceptor specific in photosynthetic by:   The book discusses biochemical, cell and structural biological, and molecular biological techniques needed for the study of photoreceptor cells.

The text also describes the methods for observing the structure of photoreceptor cells and for studying their metabolism. The procedures for photoreceptor cell isolation are also Edition: 1. Isolation and characterization of mouse-human somatic cell hybrids which produce a leukemia associat.

In this study we report the isolation and characterization of this gene, which we named C1orf The C1orf36 gene is composed of at least three exons, spanning approximately 15 kb of genomic DNA.

A transcript length of at least nucleotides is predicted for the functional C1orf36 message, encoding for a putative protein of amino by: A gene encoding a novel human 3′, 5′-cyclic nucleotide phosphodiesterase (PDE) was identified and characterized. PDE10A1 encodes a protein that is amino acids in length.

An incomplete cDNA for a second 5′-splice variant, PDE10A2, was isolated. The proteins encoded by the two variants share amino acids in by:   We used the gene knockout system (Hirochika,) to isolate the rice phyA mutants, because isolation of mutants in rice by phenotypic selection was not as easy as in Arabidopsis.

Site-directed manipulation of chromosomal genes is a powerful tool with which to investigate the in situ function of isolated by: Isolation and characterization of phytochrome is a classic example of how photobiological techniques can predict the nature of an unknown photoreceptor.

Description Isolation and characterization of a photoreceptor gene MPD1 EPUB

Current knowledge of the phytochrome photoreceptor family is given in Part 4 and that of blue/UV receptors in Part 5. Part 6 deals with the coaction of photoreceptors. The elucidation of the gene will be useful in studying the regulation of expression of X-arrestin and in screening the gene as a candidate gene for cone diseases.

This paper describes the results of the isolation and characterization of the by: Characterization of the chr1, chr2 and mat3 mutants confirmed the value of photoreceptor mutants for physiological studies.

Genes of interest were disrupted in % of preselected clones (~1 out. ResearchGate has not been able to resolve any references for this publication.

Isolation and characterization of a photoreceptor gene MPD1. Isolation and characterization of the gene. Isolation and characterization of the gene for XIP, a proposed DNA repair protein December International Journal of Radiation OncologyBiologyPhysics Randi A.

Schea. Characterization of MPP4, a gene highly expressed in photoreceptor cells, and mutation analysis in retinitis pigmentosa Author links open overlay panel Ivan Conte a b Marta Lestingi a Anneke den Hollander c Maria Giuseppina Miano b Giovanna Alfano a Diego Circolo a Mariarosaria Pugliese a Francesco Testa d Francesca Simonelli d Ernesto Rinaldi Cited by: 1.

Introduction. Phosducin (PDC) is a cytosolic phosphoprotein found in retinal photoreceptor cells (Lee et al., ; Abe et al., ) and in the pineal gland (Craft et al., ), which plays a regulatory role in the retinal phototransduction functions in the modulation of G-protein-mediated signaling processes by interacting with transducin (G protein) βγ-subunits, and thus Cited by:   The eye prototype, which is due to a purely stochastic event that assembles a photoreceptor and a pigment cell into a visual organ, requires the function of at least two classes of genes, a master control gene, Pax6, and the structural genes encoding on rhodopsin, for instance, the top and the bottom of the genetic cascade.

Starting from such a Cited by:   Phytochrome, a red/far-red-light photoreceptor protein of plants, is encoded by a small gene family. Phytochrome A (PHYA), the product of the PHYA gene, is the predominant molecular species of phytochrome in etiolated tissue and has been best characterized biochemically.

To define a role for PHYA, we isolated new mutants, designated fre1 (far-red elongated), in Arabidopsis thaliana Cited by: Protocols for photoreceptor outer segment (POS) isolation that can be used in phagocytosis assays of retinal pigment epithelium (RPE) cells have routinely used a large number of cow or pig eyes.

However, when working with large animal models (e.g., dog, cats, transgenic pigs) of inherited retinal degenerative diseases, access to retinal tissues Author: Raghavi Sudharsan, Michael H.

Elliott, Natalia Dolgova, Gustavo D. Aguirre, William A. Beltran. Isolation and characterization of PKC-L, a new member of the protein kinase C-related gene family specifically expressed in lung, skin, and heart. N Bacher, Y Zisman, E Berent, and E Livneh Department of Chemical Immunology, Weizmann Institute of Science, Rehovot, by:   Isolation of Rice phyA Mutants from Mutant Panels.

We screened a large population of Tos17 insertional mutants of rice by PCR to isolate rice phyA mutants. Three mutant panels were subjected to PCR-based screening using all combinations of four PHYA-specific and two Tosspecific a result, we identified three mutant lines, osphyA-1, osphyA-2, and osphyA-3, in which Cited by:   In plant photomorphogenesis, it is well accepted that the perception of red/far-red and blue light is mediated by distinct photoreceptor families, i.e., the phytochromes and blue-light photoreceptors, respectively.

Here we describe the discovery of a photoreceptor gene from the fern Adiantum that encodes a protein with features of both phytochrome and NPH1, the putative blue-light Cited by: Characterization of antibody against the N-terminus of RDS/peripherin. Ophthalmic Genetics19 (3), DOI: /opge Han Chang, Walter Gilbert.

Details Isolation and characterization of a photoreceptor gene MPD1 PDF

A Novel Zebrafish Gene Expressed Specifically in the Photoreceptor Cells of the by:   Genetic mutations in aryl hydrocarbon receptor interacting protein-like 1 (AIPL1) cause photoreceptor degeneration associated with Leber congenital amaurosis 4 (LCA4) in Cited by: 6. To better understand how these mutations lead to the development of disease, knowledge of the structure-function relationships of the peripherin/rds gene is facilitate these studies, genomic clones encoding the murine gene were isolated using bovine cDNA sequences as : Tong Cheng, Muna I.

Naash. Abstract. Retinal degeneration slow (rds)is a neurological mutation of mice that is characterized phenotypically by abnormal development of rod and cone photoreceptors, followed by their slow other cell types in the retina or the CNS are affected.

In rds/rds homozygotes, the retina undergoes entirely normal development and differentiation of cells until the first postnatal week Cited by: 5. Phytochrome, a red/far-red-light photoreceptor protein of plants, is encoded by a small gene family.

Phytochrome A (PHYA), the product of the PHYA gene, is the predominant molecular species of phytochrome in etiolated tissue and has been best characterized biochemically.

Download Isolation and characterization of a photoreceptor gene MPD1 FB2

The fast-growing biflagellated single-celled chlorophyte Chlamydomonas reinhardtii is the most widely used alga in basic research. The physiological functions of the 18 sensory photoreceptors are of particular interest with respect to Chlamydomonas development and behavior.

Despite the demonstration of gene editing in Chlamydomonas inthe isolation of mutants lacking easily Cited by:   Abstract. To identify genes expressed in cone photoreceptors, we previously carried out subtractive hybridization and microarrays of retinal mRNAs from normal and cd (cone degeneration) dogs.

One of the isolated genes encoded ZBED4, a novel protein that in human retina is localized to cone photoreceptors and glial Müller by: 1. We report here the isolation and characterization of an Opn4 gene from the fat-tailed dunnart.

Furthermore, both molecular and bioinformatic approaches suggest that like their placental relatives, the marsupials have retained only the Opn4m gene, having lost the Opn4x by:   Studies with mutants in four members of the five-membered Arabidopsis phytochrome (phy) family (phyA, phyB, phyD, and phyE) have revealed differential photosensory and/or physiological functions among them, but identification of a phyC mutant has proven elusive.

We now report the isolation of multiple phyC mutant alleles using reverse-genetics by: Ensembl ENSG ENSMUSG UniProt P P RefSeq (mRNA) NM_ NM_ NM_ NM_ NM_ NM_ RefSeq (protein) NP_ NP_ NP_ NP_ NP_ NP_ Location (UCSC) Chr 1: – Mb Chr 4: – Mb PubMed search Wikidata Aliases: GNB1, G protein subunit beta 1, MRD